Targeted sequencing is a sequencing method that involves enriching specific genomic regions and performing high-depth sequencing on these regions to obtain precise sequencing results. It is mainly used in tumor detection, genetic disease detection, pathogen detection, and other fields. Based on different principles of targeted capture, there are usually two methods: probe hybridization targeted capture and multiplex PCR targeted capture. In probe hybridization targeted capture, probes (DNA/RNA) are designed for the target region. These biotin-labeled probes hybridize with the target region and the biotin-labeled probes capture and enrich the target region with the help of streptavidin-coated magnetic beads. MultiplexPCR targeted capture, on the other hand, designs primers for the target region and amplifies and enriches the target region using PCR. Among the two methods, the probe hybridization targeted capture process for building a library involves many steps and takes a long time. The capture efficiency and subsequent sequencing accuracy of the target region can be affected by the temperature control and consistency of operations during the hybridization and capture steps.
Principle: Design and synthesize specific probes based on known genomic sequences, and achieve capture and enrichment of the target region through hybridization, facilitating more efficient sequencing.
DNA Fragmentation
End Repair, Adding A
Adaptor Ligation
Magnetic Bead Purification
PCR Amplification
Magnetic Bead Purification
Library Mixing
Magnetic Bead Concentration
Probe Hybridization
Targeted Capture
Post-PCR
RayKol Vitae 180 NGS Library Preparation Instrument enables the automation of library construction and hybrid capture experiments, providing solutions for efficient and stable sequencing in laboratories.
Vitae next-generation sequencing library preparation (NGSLP)
Vitae 180 NGS Library Preparation Instrument is suitable for medium-throughput (1-32 samples per run) library preparation needs. It automates the processes of library construction, hybrid capture, library normalization, library pooling, and more, reducing manual operation time and improving work efficiency while ensuring reproducibility and uniformity of results. The open platform allows for custom instrument operation processes, adapting to different manufacturers' library prep and capture kits, with one-click start for pre-matched commercial hybrid capture kit workflows, achieving standardized and validated automated experimental operations.
Vitae 180 NGS Hybrid Capture Automated Workflow
Probe Hybridization System Configuration
PCR Instrument Overnight Hybridization
Capture Reagent Washing and Thermal Incubation
Capture Enrichment and Library Washing
Post-PCR
Magnetic Bead Purification
Unique Magnetic Bead Mixing Mode (Blow-Suction + Vibration)
Fully Suspended, ensuring capture stability and improving capture efficiency.
8-channel pipetting head with single-channel function for precise small-volume liquid addition. Optional 32-channel pipetting head for high-throughput liquid handling, increasing efficiency;
Pipetting Accuracy and CV: 20-200μL accuracy ±1%, CV ≤1%; 5-20μL accuracy ±2%, CV ≤2%; 1-5μL accuracy ±5%, CV ≤5%.
Easily import initial sample concentration CSV files. The software automatically calculates the required volume of dilution solution for sample dilution, reducing manual errors and saving manpower.
Automated PCR Instrument enables accurate temperature control from 4-100℃. The PCR plate heating module allows for constant temperature incubation. The workstation follows the automated workflow setup, ensuring precise control of time and temperature, enhancing capture efficiency and uniformity.
Integrates grab plate function, PCR instrument, cooling module, PCR plate shaking module, PCR plate heating module, and magnetic attachment module for PCR plates. It completes actions such as heating, liquid addition, mixing, resting, magnetic absorption, supernatant removal, PCR amplification, avoiding efficiency loss, error-prone manual operations, and unstable library preparation results.
User-friendly software design with drag-and-drop icons, simple and easy programming, and the ability to pause, check the process, and resume experiments as needed.
Modular function design allows for custom dispensation methods, pipetting parameters, liquid handling sequences, shaking speeds and times, and more, based on functional parameter settings.
Adaptable reagent slots compatible with various library preparation and capture kit workflows. Consumables are open and suitable for multiple manufacturers' microplates, pipette tips, and reagent reservoirs.
Equipped with a UV disinfection lamp and optional positive and negative pressure HEPA filter devices based on experimental needs, effectively preventing contamination.
Consistent library output with quality control meeting sequencing requirements.
Automating and standardizing the complex library construction process for sequencing is an inevitable trend. The RayKol Vitae 180-NGS Library Preparation Instrument can effectively ensure the uniformity and stability of library quality, avoid repetitive library construction, and save manpower and economic costs.
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