Traditional sequencing is conducted on the tissue level, and the results are averages of multiple cells' genetic information, making it impossible to pinpoint the genetic information of individual cells. In reality, there is heterogeneity between cells, even within tumor tissues. The genetic information at the genome and transcriptome levels differs among cells at the tumor's center, surrounding cells, and cells that have metastasized to distant locations. Single-cell sequencing involves sequencing DNA or RNA at the single-cell level to analyze individual cells' gene structures and expressions, achieving higher resolution in gene expression analysis.
Currently, single-cell sequencing mainly includes single-cell genome sequencing, single-cell transcriptome sequencing, and single-cell epigenome sequencing.
Early single-cell sequencing processes first involved isolating single cells through dilution, flow sorting, or microdissection, followed by micro-library construction. This process was complex, had low throughput, and was costly. Today's mainstream high-throughput single-cell capture sequencing uses barcodes to label individual cells, allowing for the mixing of multiple cells for library sequencing. During subsequent data analysis, molecular information marked with the same barcode is considered to be from the same cell.
The following process, represented by Micro Fluidic Digital Droplet + Barcoded Beads technology by Yinqing Biotech, utilizes a high-throughput single-cell transcriptome library kit in conjunction with the RayKol Vitae 120 NGS-SA library construction instrument, achieving efficient cell transcriptome library construction for eight samples.
In the single-cell transcriptome sequencing process, the library construction step can be automated using the RayKol Vitae 120 NGS-SA library construction instrument.
A professional and stable automated library construction platform.
Includes all functional modules needed for library construction (PCR plate cooling slot, PCR plate heating module, PCR plate shaking module, PCR plate magnetic rack, PCR instrument), meeting the requirements of different library construction processes;
Software is modular, with no need for script writing; it adapts to different experimental processes through drag-and-drop editing;
Tested with multiple manufacturers' library construction processes (Vazyme, BGI, KAPA, Illumina, Pacbio, Nanopore) ensuring stable and reliable library construction results.
English 
한국어 
français 
русский 
português 
العربية 
Türkçe 
ไทย 
